Genetics: Fankha User and Wa Tson
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Isolation and molecular characterization of the Aspergillus nidulans wA gene.
The walls of Aspergillus nidulans conidia contain a green pigment that protects the spores from damage by ultraviolet light. At least two genes, wA and yA, are required for pigment synthesis: yA mutants produce yellow spores, wA mutants produce white spores, and wA mutations are epistatic to yA mutations. We cloned wA by genetic complementation of the wA3 mutation with a cosmid library containi...
متن کاملInsertional inactivation and cloning of the wA gene of Aspergillus nidulans.
We describe examples of wA gene inactivation (resulting in white conidiospores) obtained during transformation of Aspergillus nidulans. One wA- transformant was obtained by transformation with a prn+ plasmid of a strain with green conidia (wA+) which was unable to catabolize L-proline (prn-). This transformant contains a very large number of plasmid copies integrated at a single site inseparabl...
متن کاملCharacterization of enhancer-of-white-apricot in Drosophila melanogaster.
The white-apricot (wa) allele differs from the wild-type white gene by the presence of the retrovirus-like transposable element copia within the transcription unit. Most RNAs derived from wa have 3' termini within this insertion, and only small amounts of structurally normal RNA are produced. The activity of wa is reduced in trans by a semidominant mutation in the gene Enhancer-of-white-apricot...
متن کاملInteraction of the Enhancer of white-apricot with transposable element alleles at the white locus in Drosophila melanogaster.
The Enhancer of wa [E(wa)] mutation was shown to interact strongly with 4 of 41 tested alleles of the white (w) eye color locus. All four of the affected w alleles result from the insertion of a transposable element. E(wa) was further localized cytogenetically. The locus lies between the breakpoints of T(Y;2)L11 and T(Y;2)H137 (section 60) in 2R. The original mutation was shown to be antimorphi...
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Meena Ramchandani, MD, MPH; Ronnie Russell; Lichen Jing, PhD; Amalia Magaret, PhD; Stacy Selke, MS; Meei-Li Huang, PhD; Eric Strachan, PhD; Anna Wald, MD, MPH, FIDSA; David M. Koelle, MD; Infectious Disease, University of Washington, Seattle, WA; University of Washington, Seattle, WA; Department of Laboratory Medicine, University of Washington, Seattle, WA; Laboratory Medicine, University of Wa...
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